Hotspot

psbM-trnD-GUC

LSC · 32251-33596 · 1346 bp

Back to Jatropha

Pi 0.0238

MarkerSeek score 66.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1346
variable_sites: 31
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GCGGTAGGAACTAGAATGAACA	TCCCCGGGATTGTAGTTCAA	45.5	50.0	57.9	58.3	1483-1594 bp	1.0	64.2
psbM-trnD-GUC_p2	GCGGTAGGAACTAGAATGAACA	TTCCCCGGGATTGTAGTTCA	45.5	50.0	57.9	58.3	1484-1595 bp	1.0	64.2
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	TCCCCGGGATTGTAGTTCAA	45.0	50.0	55.8	58.3	1471-1582 bp	1.0	63.3
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	TTCCCCGGGATTGTAGTTCA	45.0	50.0	55.8	58.3	1472-1583 bp	1.0	63.3
psbM-trnD-GUC_p5	GCGGTAGGAACTAGAATGAACA	TTCAATTGGTCAGAGCACCG	45.5	50.0	57.9	58.5	1468-1579 bp	1.0	63.1