Hotspot

atpA-atpF

LSC · 12671-12728 · 58 bp

Back to Jatropha

Pi 0.0526

MarkerSeek score 83.10

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 3
pi: 0.0526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	CAACGGGTTTTCCAACAAGC	55.0	50.0	58.1	58.4	261-302 bp	1.0	76.9
atpA-atpF_p2	TACTAATCTCGTCGGCTCGA	CAACGGGTTTTCCAACAAGC	50.0	50.0	57.8	58.4	179-220 bp	1.0	76.3
atpA-atpF_p3	TTGTTCAATACGTTCGCGGA	CAACGGGTTTTCCAACAAGC	45.0	50.0	58.2	58.4	205-246 bp	1.0	76.2
atpA-atpF_p4	CGTCGGCTCGAATAGTTACC	CAACGGGTTTTCCAACAAGC	55.0	50.0	58.2	58.4	170-211 bp	1.0	76.2
atpA-atpF_p5	ATCTCGTCGGCTCGAATAGT	CAACGGGTTTTCCAACAAGC	50.0	50.0	58.4	58.4	174-215 bp	1.0	74.9