Hotspot

trnG-GCC-trnR-UCU

LSC · 9676-10486 · 811 bp

Back to Jatropha

Pi 0.101

MarkerSeek score 81.50

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 54
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.66
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	TAGCCTTCCAAGCTAGCGAT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.6	56.9	617-892 bp	1.0	68.6
trnG-GCC-trnR-UCU_p2	TAGCCTTCCAAGCTAGCGAT	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.6	57.9	623-898 bp	1.0	67.9
trnG-GCC-trnR-UCU_p3	CGTCGACTATAACCCCTAGC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.0	56.9	633-908 bp	1.0	65.3
trnG-GCC-trnR-UCU_p4	CCCCTAGCCTTCCAAGCTA	AGAAGACCTCTGTCCTATCCA	57.9	47.6	58.1	56.9	621-896 bp	1.0	64.7
trnG-GCC-trnR-UCU_p5	CGTCGACTATAACCCCTAGC	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	57.0	57.9	639-914 bp	1.0	64.6