Hotspot

rps19

LSC · 89473-89823 · 351 bp

Back to Jasminum

Pi 0.0316

MarkerSeek score 72.70

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 351
variable_sites: 41
pi: 0.0316
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TAGGAGCGTCCACGAATTTG	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.0	58.1	80-643 bp	1.0	91.2
rps19_p2	TAGGAGCGTCCACGAATTTG	ATCCCAAAACTGCTCAGCAA	50.0	45.0	58.0	58.0	80-896 bp	1.0	90.6
rps19_p3	GAATGGTGACCTTGGGTTGT	ATCCCAAAACTGCTCAGCAA	50.0	45.0	58.0	58.0	80-1304 bp	1.0	90.6
rps19_p4	AGCGTCCACGAATTTGATCA	ATCCCAAAACTGCTCAGCAA	45.0	45.0	57.9	58.0	80-892 bp	1.0	89.9
rps19_p5	TGCTGCTGCAGAATAAACCA	ATCCCAAAACTGCTCAGCAA	45.0	45.0	58.1	58.0	80-978 bp	0.857143	82.8