Hotspot

rps15-ycf1

SSC · 132598-133012 · 415 bp

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Pi 0.0661
MarkerSeek score 85.00
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
415
variable_sites
89
pi
0.0661
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.98
markerseek_score
85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 GACCCTCTGTTTTCTTTGTTTTCT TGGCAGTCGTTTCAGTATGT 37.5 45.0 57.8 57.2 80-561 bp 0.928571 49.1
rps15-ycf1_p2 ACCCTCTGTTTTCTTTGTTTTCT TGGCAGTCGTTTCAGTATGT 34.8 45.0 56.7 57.2 80-560 bp 0.928571 49.1
rps15-ycf1_p3 GACCCTCTGTTTTCTTTGTT TGGCAGTCGTTTCAGTATGT 40.0 45.0 53.6 57.2 80-561 bp 0.928571 49.1
rps15-ycf1_p4 GACCCTCTGTTTTCTTTGTTT TGGCAGTCGTTTCAGTATGT 38.1 45.0 54.5 57.2 80-561 bp 0.928571 49.1
rps15-ycf1_p5 GACCCTCTGTTTTCTTTGTTTTCT TGGCAGTCGTTTCAGTATGTT 37.5 42.9 57.8 57.9 80-561 bp 0.928571 49.1