Hotspot

trnI-CAU-ycf2

IRb · 91868-92019 · 152 bp

Back to Jasminum

Pi 0.0711

MarkerSeek score 74.20

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 152
variable_sites: 40
pi: 0.0711
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	ATTGGCATGCATCCAGTAGG	TGCGCGTGAAAGTAGGATAC	50.0	50.0	58.0	58.1	80-602 bp	1.0	91.3
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	TGCGCGTGAAAGTAGGATAC	50.0	50.0	58.0	58.1	80-553 bp	1.0	91.3
trnI-CAU-ycf2_p3	ATTGGCATGCATCCAGTAGG	GTGCGCGTGAAAGTAGGATA	50.0	50.0	58.0	58.1	80-603 bp	0.928571	88.4
trnI-CAU-ycf2_p4	GTTGGGCGCTTTAACCATTC	GTGCGCGTGAAAGTAGGATA	50.0	50.0	58.0	58.1	80-554 bp	0.928571	88.4
trnI-CAU-ycf2_p5	ATTGGCATGCATCCAGTAGG	CCCGTTCTTTGTTCGGATCT	50.0	50.0	58.0	57.9	80-387 bp	0.857143	85.5