Hotspot

psaJ-rpl33

LSC · 72717-73767 · 1051 bp

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Pi 0.0413

MarkerSeek score 80.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 1051
variable_sites: 141
pi: 0.0413
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCGGATGCTTTGACA	CATCTTTCCCCTTGGCCAT	45.0	52.6	58.0	57.4	80-1102 bp	1.0	78.9
psaJ-rpl33_p2	TGTGGCACCAGTCCTAAGTA	CATCTTTCCCCTTGGCCAT	50.0	52.6	58.0	57.4	80-1170 bp	1.0	78.9
psaJ-rpl33_p3	TCCCGGATGCTTTGACATTT	CATCTTTCCCCTTGGCCAT	45.0	52.6	57.8	57.4	80-1099 bp	1.0	77.3
psaJ-rpl33_p4	TTCCCGGATGCTTTGACATT	CATCTTTCCCCTTGGCCAT	45.0	52.6	57.8	57.4	80-1100 bp	1.0	77.3
psaJ-rpl33_p5	TTTCCCGGATGCTTTGACAT	CATCTTTCCCCTTGGCCAT	45.0	52.6	57.8	57.4	80-1101 bp	1.0	77.3