Hotspot

ycf3-cemA

LSC · 65329-66252 · 924 bp

Back to Jasminum

Pi 0.0192

MarkerSeek score 78.40

Primer available yes

Label ycf3-cemA

Feature Metadata

label_name: ycf3-cemA
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 75
pi: 0.0192
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-cemA_p1	TTGAAGATCACGAGGCGTTT	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	80-1090 bp	1.0	88.5
ycf3-cemA_p2	TTGGTTGAAGATCACGAGGC	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.2	58.1	80-1094 bp	1.0	88.3
ycf3-cemA_p3	TGGTTTGATCAAGCAGCTGA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.7	58.1	80-1168 bp	1.0	87.5
ycf3-cemA_p4	GGAAACAGGCTATAGCGCTT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	80-1136 bp	0.928571	86.3
ycf3-cemA_p5	GGAAACAGGCTATAGCGCTT	CCACCAGGGCAAAAAGACTA	50.0	50.0	58.0	57.7	80-1129 bp	0.928571	84.6