Hotspot

trnS-GGA-ycf3

LSC · 62529-63348 · 820 bp

Back to Jasminum

Pi 0.0127

MarkerSeek score 77.60

Primer available yes

Label trnS-GGA-ycf3

Feature Metadata

label_name: trnS-GGA-ycf3
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 48
pi: 0.0127
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-ycf3_p1	CCCTCGGTAAACAAAAGCCT	CATTTATCCGCGATCTAGGCA	50.0	47.6	58.1	58.0	80-970 bp	1.0	79.4
trnS-GGA-ycf3_p2	ACGGAAAGAGAGGGATTCGA	CATTTATCCGCGATCTAGGCA	50.0	47.6	58.2	58.0	80-991 bp	1.0	78.9
trnS-GGA-ycf3_p3	TTCCAATGCTACGCCTTGAA	CATTTATCCGCGATCTAGGCA	45.0	47.6	57.8	58.0	80-941 bp	1.0	78.8
trnS-GGA-ycf3_p4	ACCCTCGGTAAACAAAAGCC	CATTTATCCGCGATCTAGGCA	50.0	47.6	58.4	58.0	80-971 bp	1.0	77.1
trnS-GGA-ycf3_p5	CCCTCGGTAAACAAAAGCCT	TTTATCCGCGATCTAGGCAT	50.0	45.0	58.1	56.6	80-968 bp	1.0	76.4