Hotspot

psaI-trnI-CAU

LSC · 47409-49197 · 1789 bp

Back to Jasminum

Pi 0.0937

MarkerSeek score 83.50

Primer available yes

Label psaI-trnI-CAU

Feature Metadata

label_name: psaI-trnI-CAU
feature_type: igs
region: LSC
length_bp: 1789
variable_sites: 531
pi: 0.0937
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-trnI-CAU_p1	ACAGATGAGATTGGGTCCCA	GGGGAGTGACATTGATCCAC	50.0	55.0	58.0	58.0	1941-2824 bp	0.642857	77.1
psaI-trnI-CAU_p2	TTTTAGCCATCCAGCGATCC	GGGGAGTGACATTGATCCAC	50.0	55.0	58.0	58.0	2153-2996 bp	0.571429	74.2
psaI-trnI-CAU_p3	GCCATTGCAATTGTCGGAAA	GTTGGGCGCTTTAACCATTC	45.0	50.0	57.9	58.0	80-2939 bp	0.5	71.3
psaI-trnI-CAU_p4	ACAGATGAGATTGGGTCCCA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	1941-2868 bp	0.357143	66.0
psaI-trnI-CAU_p5	TTTTAGCCATCCAGCGATCC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	2145-2672 bp	0.357143	66.0