Hotspot

trnG-GCC-trnfM-CAU

LSC · 40058-40245 · 188 bp

Back to Jasminum

Pi 0.0535

MarkerSeek score 78.10

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 34
pi: 0.0535
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	GTTCAAATCCTGTCTCCGCA	50.0	50.0	58.1	58.2	232-241 bp	1.0	90.0
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	GTTCAAATCCTGTCTCCGCA	50.0	50.0	58.1	58.2	233-242 bp	1.0	90.0
trnG-GCC-trnfM-CAU_p3	AGAAGATGCGGGTTCGATTC	GTAGCTCGCAAGGCTCATAA	50.0	50.0	58.1	57.8	266-275 bp	1.0	89.7
trnG-GCC-trnfM-CAU_p4	AGAAGATGCGGGTTCGATTC	TAGCTCGCAAGGCTCATAAC	50.0	50.0	58.1	57.8	265-274 bp	1.0	89.7
trnG-GCC-trnfM-CAU_p5	GAGAAGATGCGGGTTCGATT	TAGCTCGCAAGGCTCATAAC	50.0	50.0	58.1	57.8	266-275 bp	1.0	89.7