Hotspot

psbZ-trnG-GCC

LSC · 39585-39986 · 402 bp

Back to Jasminum

Pi 0.0327

MarkerSeek score 74.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 50
pi: 0.0327
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	GGGTTAGTCTTTCTGGTGGG	AATCGAACCCGCATCTTCTC	55.0	50.0	57.5	58.1	463-513 bp	1.0	87.6
psbZ-trnG-GCC_p2	GGGTTAGTCTTTCTGGTGGG	GAATCGAACCCGCATCTTCT	55.0	50.0	57.5	58.1	464-514 bp	1.0	87.6
psbZ-trnG-GCC_p3	GGGTTAGTCTTTCTGGTGGG	GAACCCGCATCTTCTCCTTG	55.0	55.0	57.5	58.6	459-509 bp	1.0	83.1
psbZ-trnG-GCC_p4	AGTCTTTCTGGTGGGTATCCT	AATCGAACCCGCATCTTCTC	47.6	50.0	57.8	58.1	458-508 bp	1.0	81.9
psbZ-trnG-GCC_p5	AGTCTTTCTGGTGGGTATCCT	GAATCGAACCCGCATCTTCT	47.6	50.0	57.8	58.1	459-509 bp	1.0	81.9