Hotspot

trnG-UCC-trnR-UCU

LSC · 11239-11416 · 178 bp

Back to Jasminum

Pi 0.0625

MarkerSeek score 84.70

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 44
pi: 0.0625
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGGAAAGAAATTGCGTCCAA	50.0	40.0	58.0	56.2	295-318 bp	1.0	77.0
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	TGGAAAGAAATTGCGTCCAA	55.0	40.0	58.1	56.2	298-321 bp	1.0	76.8
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	248-271 bp	1.0	75.3
trnG-UCC-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	ATGGAAAGAAATTGCGTCCA	50.0	40.0	58.0	55.9	296-319 bp	1.0	75.1
trnG-UCC-trnR-UCU_p5	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	251-274 bp	1.0	75.1