Hotspot

trnH-GUG-psbA

LSC · 92-552 · 461 bp

Back to Jasminum

Pi 0.0353

MarkerSeek score 76.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 461
variable_sites: 67
pi: 0.0353
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	AATCCACTGCCTTAATCCGC	GAAGCTCCATCTACAAATGG	50.0	45.0	58.3	53.8	506-535 bp	1.0	55.5
trnH-GUG-psbA_p2	CCTTAATCCGCTTGGCTACA	GAAGCTCCATCTACAAATGG	50.0	45.0	57.7	53.8	497-526 bp	1.0	55.5
trnH-GUG-psbA_p3	ATGAGTTATGGGCGAACGAC	GAAGCTCCATCTACAAATGG	50.0	45.0	58.1	53.8	80-585 bp	0.928571	54.7
trnH-GUG-psbA_p4	CGCATGGTGGATTCACAATC	GAAGCTCCATCTACAAATGG	50.0	45.0	57.5	53.8	522-551 bp	1.0	54.2
trnH-GUG-psbA_p5	TAATCCGCTTGGCTACATCC	GAAGCTCCATCTACAAATGG	50.0	45.0	57.5	53.8	494-523 bp	1.0	53.7