Hotspot

rps16

LSC · 4494-5586 · 1093 bp

Back to Jansenella

Pi 0.00183

MarkerSeek score 59.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1093
variable_sites: 2
pi: 0.00183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GCACGACATAAATGCAGCAA	TGGATCAATTCTCGATACCA	45.0	40.0	57.7	53.5	1257 bp	1.0	42.4
rps16_p2	GCACGACATAAATGCAGCAA	TTGGATCAATTCTCGATACCA	45.0	38.1	57.7	54.4	1258 bp	1.0	41.5
rps16_p3	GCACGACATAAATGCAGCAA	TGGATCAATTCTCGATACCAT	45.0	38.1	57.7	54.2	1257 bp	1.0	40.1
rps16_p4	GCACGACATAAATGCAGCAA	AGCAATGACTATTCATGATTCCA	45.0	34.8	57.7	56.0	1287 bp	1.0	40.1
rps16_p5	GCACGACATAAATGCAGCAA	TGGATCAATTCTCGATACCATT	45.0	36.4	57.7	55.0	1257 bp	1.0	40.1