Hotspot

rpoA

LSC · 75639-76664 · 1026 bp

Back to Jansenella

Pi 0.0039

MarkerSeek score 61.00

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1026
variable_sites: 4
pi: 0.0039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AGATTGTGCCAAAGATGCAA	ACACCTATGCCGCATAATGG	40.0	50.0	56.2	58.1	1225 bp	1.0	65.1
rpoA_p2	AGATTGTGCCAAAGATGCAA	CGCATAATGGATGTCGACCT	40.0	50.0	56.2	57.8	1215 bp	1.0	64.7
rpoA_p3	AGATTGTGCCAAAGATGCAA	CACCTATGCCGCATAATGGA	40.0	50.0	56.2	57.8	1224 bp	1.0	64.4
rpoA_p4	AGATTGTGCCAAAGATGCAAA	ACACCTATGCCGCATAATGG	38.1	50.0	57.0	58.1	1225 bp	1.0	63.2
rpoA_p5	AGATTGTGCCAAAGATGCAAA	CGCATAATGGATGTCGACCT	38.1	50.0	57.0	57.8	1215 bp	1.0	62.8