Hotspot

psbC

LSC · 9596-11017 · 1422 bp

Back to Jansenella

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 2
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTCGTTTCCCAGGAAATCCG	GAATGGCTCGGCTAAATGGA	50.0	50.0	58.2	58.0	1609 bp	1.0	78.4
psbC_p2	GCAGCGGAAGATCCTGAATT	GAATGGCTCGGCTAAATGGA	50.0	50.0	58.3	58.0	1588 bp	1.0	77.2
psbC_p3	TTCGTTTCCCAGGAAATCCG	TCCTTGTTCAGCCCCCTTAT	50.0	50.0	58.2	58.3	1653 bp	1.0	76.1
psbC_p4	TTCGTTTCCCAGGAAATCCG	TGAATGGCTCGGCTAAATGG	50.0	50.0	58.2	58.3	1610 bp	1.0	76.1
psbC_p5	TTCGTTTCCCAGGAAATCCG	ACCGAGCCAGAACAGAAAAA	50.0	45.0	58.2	57.7	1586 bp	1.0	75.9