Hotspot

psbA

LSC · 88-1149 · 1062 bp

Back to Jansenella

Pi 0.000942

MarkerSeek score 49.20

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TTTCCGGCTTTCCTTCCTTC	TATGTGTGCTTGGGAGTCCT	50.0	50.0	58.1	58.3	1159 bp	1.0	76.6
psbA_p2	GGGTATTTCCGGCTTTCCTT	TATGTGTGCTTGGGAGTCCT	50.0	50.0	57.9	58.3	1164 bp	1.0	76.2
psbA_p3	TTGGGTATTTCCGGCTTTCC	TATGTGTGCTTGGGAGTCCT	50.0	50.0	58.2	58.3	1166 bp	1.0	76.1
psbA_p4	CCGGCTTTCCTTCCTTCAAA	TATGTGTGCTTGGGAGTCCT	50.0	50.0	58.4	58.3	1156 bp	1.0	74.3
psbA_p5	TTTCCGGCTTTCCTTCCTTC	TGGGAGTCCTTGCAATTTGA	50.0	45.0	58.1	57.3	1149 bp	1.0	73.9