Hotspot

petB

LSC · 72574-74005 · 1432 bp

Back to Jansenella

Pi 0.000707

MarkerSeek score 60.30

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1432
variable_sites: 1
pi: 0.000707
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGTAGTTCGACCGTGGATTT	ACCACAACCTACCCGATATGA	50.0	47.6	57.6	58.2	1516-1532 bp	1.0	68.0
petB_p2	GGTAGTTCGACCGTGGATTT	ACCACAACCTACCCGATATG	50.0	50.0	57.6	56.7	1516-1532 bp	1.0	67.6
petB_p3	GGTAGTTCGACCGTGGATTT	TGAAATACCACAACCTACCCG	50.0	47.6	57.6	57.7	1522-1538 bp	1.0	67.2
petB_p4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1521-1537 bp	1.0	65.8
petB_p5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1522-1538 bp	1.0	65.8