Hotspot

ndhH

SSC · 116044-117225 · 1182 bp

Back to Jansenella

Pi 0.00169
MarkerSeek score 59.70
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
SSC
length_bp
1182
variable_sites
2
pi
0.00169
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 TCCCAGTAGGAGGGTCAAAA TTGCATTTTGATGAGCCTCA 50.0 40.0 57.6 55.9 1369 bp 1.0 60.0
ndhH_p2 TCCCAGTAGGAGGGTCAAAA GAACAAGGACGAAACGAAAGA 50.0 42.9 57.6 56.8 1320 bp 1.0 59.1
ndhH_p3 TCCCAGTAGGAGGGTCAAAA AGAACAAGGACGAAACGAAAGA 50.0 40.9 57.6 58.2 1321 bp 1.0 59.0
ndhH_p4 TCCCAGTAGGAGGGTCAAAA AGAACAAGGACGAAACGAAAG 50.0 42.9 57.6 56.8 1321 bp 1.0 59.0
ndhH_p5 TCCCAGTAGGAGGGTCAAAA TTTGCATTTTGATGAGCCTCA 50.0 38.1 57.6 56.7 1370 bp 1.0 58.2