Hotspot

ccsA

SSC · 108514-109482 · 969 bp

Back to Jansenella

Pi 0.00206

MarkerSeek score 59.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 2
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGCATTCTCGAAAAAGAA	GACGTTCAAGGGGTTCTCAA	45.0	50.0	57.4	57.8	1148 bp	1.0	73.2
ccsA_p2	GCGGCATTCTCGAAAAAGAA	AAGACGTTCAAGGGGTTCTC	45.0	50.0	57.4	57.5	1150 bp	1.0	70.9
ccsA_p3	GCGGCATTCTCGAAAAAGAA	AGACGTTCAAGGGGTTCTCA	45.0	50.0	57.4	58.6	1149 bp	1.0	70.6
ccsA_p4	GCGGCATTCTCGAAAAAGAA	ACGTTCAAGGGGTTCTCAAA	45.0	45.0	57.4	57.3	1147 bp	1.0	69.5
ccsA_p5	GCGGCATTCTCGAAAAAGAA	TGAAAAGACGTTCAAGGGGT	45.0	45.0	57.4	57.3	1154 bp	1.0	69.5