Hotspot

rbcL-psaI

LSC · 57494-58394 · 901 bp

Back to Jansenella

Pi 0.00111

MarkerSeek score 59.50

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 1
pi: 0.00111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GCAAATGGAGCCCTGAACTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	1056-1057 bp	1.0	78.9
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1036-1037 bp	1.0	78.6
rbcL-psaI_p3	GCAAATGGAGCCCTGAACTA	AAGGAAGCCATTGCAATTGC	50.0	45.0	57.9	57.9	1063-1064 bp	1.0	78.3
rbcL-psaI_p4	GCCGCAGCTTGTGAAATATG	AAGGAAGCCATTGCAATTGC	50.0	45.0	58.2	57.9	1043-1044 bp	1.0	78.0
rbcL-psaI_p5	GAGTTCAAGCCGGTGGATAC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.3	58.0	1000-1001 bp	1.0	77.2