Hotspot

ycf3-trnS-GGA

LSC · 45489-46080 · 592 bp

Back to Jansenella

Pi 0.00338

MarkerSeek score 60.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 2
pi: 0.00338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	AAATGCCCTCTTTTCTCCGG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.2	58.2	788 bp	1.0	77.7
ycf3-trnS-GGA_p2	AAATGCCCTCTTTTCTCCGG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.2	57.8	738 bp	1.0	77.4
ycf3-trnS-GGA_p3	TTGGCTACAATCGAGGAGGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.4	58.2	739 bp	1.0	75.6
ycf3-trnS-GGA_p4	TTGGCTACAATCGAGGAGGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.4	57.8	689 bp	1.0	75.2
ycf3-trnS-GGA_p5	AAATGCCCTCTTTTCTCCGG	AGTTCCAATGCTACGCCTTG	50.0	50.0	58.2	58.5	740 bp	1.0	74.6