Hotspot

trnC-GCA-rpoB

LSC · 19397-20586 · 1190 bp

Back to Jansenella

Pi 0.00253

MarkerSeek score 55.30

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1190
variable_sites: 3
pi: 0.00253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ACCCCTCGAATTGGATCTGA	50.0	50.0	58.1	58.1	1294-1300 bp	1.0	79.0
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CCCCTCGAATTGGATCTGAC	50.0	55.0	58.1	57.8	1293-1299 bp	1.0	78.0
trnC-GCA-rpoB_p3	CTGGGGGTAAAGGATTTGCA	ACCCCTCGAATTGGATCTGA	50.0	50.0	57.8	58.1	1302-1308 bp	1.0	77.7
trnC-GCA-rpoB_p4	TGGGGGTAAAGGATTTGCAG	ACCCCTCGAATTGGATCTGA	50.0	50.0	57.8	58.1	1301-1307 bp	1.0	77.7
trnC-GCA-rpoB_p5	GGGGGTAAAGGATTTGCAGT	ACCCCTCGAATTGGATCTGA	50.0	50.0	57.8	58.1	1300-1306 bp	1.0	77.7