Hotspot

petN-trnC-GCA

LSC · 18402-19325 · 924 bp

Back to Jansenella

Pi 0.00108
MarkerSeek score 59.40
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
924
variable_sites
1
pi
0.00108
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGTCCACTCCTTCCCCATAC CTGCAAATCCTTTACCCCCA 55.0 50.0 58.1 57.8 1051 bp 1.0 77.3
petN-trnC-GCA_p2 AGTCCACTCCTTCCCCATAC TGCAAATCCTTTACCCCCAG 55.0 50.0 58.1 57.8 1050 bp 1.0 77.3
petN-trnC-GCA_p3 GTCCACTCCTTCCCCATACT CTGCAAATCCTTTACCCCCA 55.0 50.0 58.1 57.8 1050 bp 1.0 77.3
petN-trnC-GCA_p4 GTCCACTCCTTCCCCATACT TGCAAATCCTTTACCCCCAG 55.0 50.0 58.1 57.8 1049 bp 1.0 77.3
petN-trnC-GCA_p5 GTCCACTCCTTCCCCATACT GCAAATCCTTTACCCCCAGT 55.0 50.0 58.1 57.8 1048 bp 1.0 77.3