Hotspot

trnG-GCC-trnfM-CAU

LSC · 12132-12550 · 419 bp

Back to Jansenella

Pi 0.0119

MarkerSeek score 61.80

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 419
variable_sites: 5
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GGTCGACCCAGGTGGATATA	CGAGGCTCATAACCTTGAGG	55.0	55.0	58.0	57.8	578-579 bp	1.0	78.8
trnG-GCC-trnfM-CAU_p2	TCGACCCAGGTGGATATACC	CGAGGCTCATAACCTTGAGG	55.0	55.0	58.0	57.8	576-577 bp	1.0	78.8
trnG-GCC-trnfM-CAU_p3	TCGACCCAGGTGGATATACC	CAGTTTGGTAGCTCACGAGG	55.0	55.0	58.0	58.3	591-592 bp	1.0	78.4
trnG-GCC-trnfM-CAU_p4	GGTCGACCCAGGTGGATATA	CAGTTTGGTAGCTCACGAGG	55.0	55.0	58.0	58.3	593-594 bp	1.0	78.3
trnG-GCC-trnfM-CAU_p5	TCGACCCAGGTGGATATACC	TACAGAGCGGAGTAGAGCAG	55.0	55.0	58.0	58.3	608-609 bp	1.0	78.0