Hotspot

psbZ-trnG-GCC

LSC · 11795-12073 · 279 bp

Back to Jansenella

Pi 0

MarkerSeek score 32.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 279
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	TCGAACCCGTATCTTCTCCT	50.0	50.0	57.8	57.6	432 bp	1.0	74.9
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTC	TCGAACCCGTATCTTCTCCT	55.0	50.0	58.3	57.6	430 bp	1.0	74.5
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	CCGTATCTTCTCCTTGGCAA	50.0	50.0	57.8	57.3	426 bp	1.0	72.8
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	CCGTATCTTCTCCTTGGCAA	55.0	50.0	58.3	57.3	424 bp	1.0	72.4
psbZ-trnG-GCC_p5	TTGCTTCTCCTGATGGTTGG	GGGAATCGAACCCGTATCTT	50.0	50.0	57.8	57.1	437 bp	1.0	71.1