Hotspot

trnS-GCU-psbD

LSC · 7614-8586 · 973 bp

Back to Jansenella

Pi 0.00103

MarkerSeek score 59.30

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 973
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	AAAACGGTCCCTTCGTAACC	45.0	50.0	58.0	58.1	1097-1102 bp	1.0	78.8
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1118-1123 bp	1.0	78.4
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	AAACAAAACGGTCCCTTCGT	45.0	45.0	58.0	58.2	1101-1106 bp	1.0	77.8
trnS-GCU-psbD_p4	GCTTTAGTCCACTCAGCCAT	AAAACGGTCCCTTCGTAACC	50.0	50.0	57.6	58.1	1083-1088 bp	1.0	75.9
trnS-GCU-psbD_p5	GCTTTAGTCCACTCAGCCAT	GCCGGACCATCCTACAAAAA	50.0	50.0	57.6	58.2	1104-1109 bp	1.0	75.4