Hotspot

trnN-GUU-trnR-ACG

SSC · 131629-132206 · 578 bp

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Pi 0.00692

MarkerSeek score 62.30

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: SSC
length_bp: 578
variable_sites: 4
pi: 0.00692
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	670 bp	1.0	77.5
trnN-GUU-trnR-ACG_p2	TGGGAACGGGAATTGAACTC	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	755 bp	1.0	77.1
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	50.0	55.0	58.2	57.7	707 bp	1.0	76.9
trnN-GUU-trnR-ACG_p4	TGGGAACGGGAATTGAACTC	GAACAGATCCAGTGGAGACG	50.0	55.0	57.8	57.7	792 bp	1.0	76.6
trnN-GUU-trnR-ACG_p5	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	674 bp	1.0	76.6