Hotspot

rps15-ycf1

SSC · 125168-129107 · 3940 bp

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Pi 0.00127

MarkerSeek score 54.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 3940
variable_sites: 5
pi: 0.00127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAAAACGGTGGTAGGTACGT	TGGTAGCCGTTTCAGTATCT	50.0	45.0	57.8	56.0	276 bp	1.0	63.4
rps15-ycf1_p2	CAAAACGGTGGTAGGTACGT	TGGTAGCCGTTTCAGTATCTT	50.0	42.9	57.8	56.7	276 bp	1.0	61.6
rps15-ycf1_p3	CCAAAACGGTGGTAGGTACG	TGGTAGCCGTTTCAGTATCT	55.0	45.0	58.6	56.0	277 bp	1.0	60.1
rps15-ycf1_p4	CAAAACGGTGGTAGGTACGT	ATGGTAGCCGTTTCAGTATCT	50.0	42.9	57.8	56.5	277 bp	1.0	59.8
rps15-ycf1_p5	CAAAACGGTGGTAGGTACGT	CCAATAATGGTAGCCGTTTCAG	50.0	45.5	57.8	57.4	283 bp	1.0	59.2