Hotspot

rpl32-sprA

SSC · 114969-115011 · 43 bp

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Pi 0.0233

MarkerSeek score 65.90

Primer available yes

Label rpl32-sprA

Feature Metadata

label_name: rpl32-sprA
feature_type: igs
region: SSC
length_bp: 43
variable_sites: 1
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-sprA_p1	AGGAAGGGATATTGGGTAGCA	TCAGAAAAGCGAATAGAGTTCCA	47.6	39.1	57.9	57.9	245-246 bp	1.0	46.5
rpl32-sprA_p2	AGCGAAATCTCTTTCTACCGG	TCAGAAAAGCGAATAGAGTTCCA	47.6	39.1	57.8	57.9	207-208 bp	1.0	45.9
rpl32-sprA_p3	AGGAAGGGATATTGGGTAGCA	CAGAAAAGCGAATAGAGTTCCA	47.6	40.9	57.9	56.5	244-245 bp	1.0	43.4
rpl32-sprA_p4	AGCGAAATCTCTTTCTACCGG	CAGAAAAGCGAATAGAGTTCCA	47.6	40.9	57.8	56.5	206-207 bp	1.0	42.7
rpl32-sprA_p5	GGAAGGGATATTGGGTAGCA	TCAGAAAAGCGAATAGAGTTCCA	50.0	39.1	56.4	57.9	244-245 bp	1.0	42.0