Hotspot

psbB-psbT

LSC · 76390-76586 · 197 bp

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Pi 0.0152

MarkerSeek score 61.30

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 3
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	ACAAAAAGACAAGCAGCCTG	GGAATTTTAGGCGGTTCTCG	45.0	50.0	57.1	56.9	307 bp	0.5	44.1
psbB-psbT_p2	ACAAAAAGACAAGCAGCCTG	GGAATTTTAGGCGGTTCTCGA	45.0	47.6	57.1	58.4	307 bp	0.5	42.0
psbB-psbT_p3	CAAAAAGACAAGCAGCCTGA	GGAATTTTAGGCGGTTCTCG	45.0	50.0	56.8	56.9	306 bp	0.5	41.8
psbB-psbT_p4	GGTGCTAGAACCTTGTTCAGA	GGAATTTTAGGCGGTTCTCG	47.6	50.0	57.6	56.9	406 bp	0.5	39.9
psbB-psbT_p5	ACAAAAAGACAAGCAGCCTG	TGGAATTTTAGGCGGTTCTCG	45.0	47.6	57.1	58.6	308 bp	0.5	39.8