Hotspot

petA-psbJ

LSC · 65238-66328 · 1091 bp

Back to Jaltomata

Pi 0.00275

MarkerSeek score 60.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1091
variable_sites: 3
pi: 0.00275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCGGTTATTTTGGCAC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.1	57.6	1202 bp	1.0	76.6
petA-psbJ_p2	GAGAAGGTTCAATTGGCCGA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1150 bp	1.0	75.9
petA-psbJ_p3	TCGAGAAGGTTCAATTGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1152 bp	1.0	75.9
petA-psbJ_p4	TGTTCTTCTTGGCATCGGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1212 bp	1.0	75.1
petA-psbJ_p5	TTGTTCTTCTTGGCATCGGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1213 bp	1.0	75.1