Hotspot

accD-psaI

LSC · 60771-61505 · 735 bp

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Pi 0.00816

MarkerSeek score 63.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 6
pi: 0.00816
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGTTTATTCGACC	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.4	57.9	930-941 bp	1.0	66.7
accD-psaI_p2	AGTTATTTAAGCTCCACGCCT	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.4	57.9	870-881 bp	1.0	66.7
accD-psaI_p3	CCAGAAGGGTTTATTCGACCT	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.4	57.9	929-940 bp	1.0	66.7
accD-psaI_p4	TCGACCTAATTGTACCACGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.3	57.9	915-926 bp	1.0	66.2
accD-psaI_p5	TCCAGAAGGGTTTATTCGACC	TGCAATTGCCGGAAAGACTA	47.6	45.0	57.4	57.8	920-931 bp	1.0	66.0