Hotspot

ycf3-trnS-GGA

LSC · 45726-46562 · 837 bp

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Pi 0.00478

MarkerSeek score 61.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 837
variable_sites: 4
pi: 0.00478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	GCGAAGGTACGGAAAGAGAG	45.0	55.0	56.4	58.1	1029 bp	1.0	66.9
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	GCGAAGGTACGGAAAGAGAG	45.0	55.0	56.4	58.1	1030 bp	1.0	66.9
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1000 bp	1.0	66.9
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1001 bp	1.0	66.9
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	1021 bp	1.0	66.4