Hotspot

ycf1

SSC · 121800-126872 · 5073 bp

Back to Jacobaea

Pi 0.386
MarkerSeek score 75.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5073
variable_sites
1743
pi
0.386
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCTTCTTCCGCCTGTGAAAA GACCGTCTCAAGGAAATCCC 45.0 55.0 57.4 58.0 904 bp 0.5 54.7
ycf1_p2 TCTTCTTCCGCCTGTGAAAA GGACCGTCTCAAGGAAATCC 45.0 55.0 57.4 58.0 905 bp 0.5 54.7
ycf1_p3 TCTTCTTCCGCCTGTGAAAA GGGACCGTCTCAAGGAAATC 45.0 55.0 57.4 58.0 906 bp 0.5 54.7
ycf1_p4 TTCTTCTTCCGCCTGTGAAA GACCGTCTCAAGGAAATCCC 45.0 55.0 57.4 58.0 905 bp 0.5 54.7
ycf1_p5 TTCTTCTTCCGCCTGTGAAA GGACCGTCTCAAGGAAATCC 45.0 55.0 57.4 58.0 906 bp 0.5 54.7