Hotspot

rps15

SSC · 121314-121592 · 279 bp

Back to Jacobaea

Pi 0.502

MarkerSeek score 77.10

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 279
variable_sites: 131
pi: 0.502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TGAGGTAAATCAGCAGCGAC	TACCGAAACCCCAAAAACGT	50.0	45.0	58.0	58.0	1494 bp	0.5	59.5
rps15_p2	TGAGGTAAATCAGCAGCGAC	AATTATGGAACCTGCCCGTC	50.0	50.0	58.0	57.9	1603 bp	0.5	59.5
rps15_p3	TGAGGTAAATCAGCAGCGAC	TATGGAACCTGCCCGTCTAT	50.0	50.0	58.0	57.9	1600 bp	0.5	59.1
rps15_p4	AACACCATGCATTGATGGGT	TACCGAAACCCCAAAAACGT	45.0	45.0	58.1	58.0	1063 bp	0.5	59.1
rps15_p5	AACACCATGCATTGATGGGT	AATTATGGAACCTGCCCGTC	45.0	50.0	58.1	57.9	1172 bp	0.5	59.1