Hotspot

psaC

SSC · 115312-115557 · 246 bp

Back to Jacobaea

Pi 0.429

MarkerSeek score 76.90

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 96
pi: 0.429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	CTCCCGCCGTGTACAAAATA	TGGCACTGTCAATCCAGAAG	50.0	50.0	58.0	57.8	1020 bp	0.5	58.4
psaC_p2	CTCCCGCCGTGTACAAAATA	GGCACTGTCAATCCAGAAGT	50.0	50.0	58.0	57.8	1019 bp	0.5	58.4
psaC_p3	ACCTGTGCCCCAAAATAAGG	TGGCACTGTCAATCCAGAAG	50.0	50.0	58.1	57.8	469 bp	0.5	58.0
psaC_p4	CCTGTGCCCCAAAATAAGGT	TGGCACTGTCAATCCAGAAG	50.0	50.0	58.1	57.8	468 bp	0.5	58.0
psaC_p5	CCTGTGCCCCAAAATAAGGT	GGCACTGTCAATCCAGAAGT	50.0	50.0	58.1	57.8	467 bp	0.5	58.0