Hotspot

ndhG

SSC · 116334-116864 · 531 bp

Back to Jacobaea

Pi 0.507

MarkerSeek score 80.80

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 242
pi: 0.507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CTTCTGGATTGACAGTGCCA	TTGATCACATCCGAGCGTTT	50.0	45.0	57.8	57.9	1920 bp	0.5	57.7
ndhG_p2	ACTTCTGGATTGACAGTGCC	TTGATCACATCCGAGCGTTT	50.0	45.0	57.8	57.9	1921 bp	0.5	57.7
ndhG_p3	CTTCTGGATTGACAGTGCCA	TAGGTGACGAGAATCCCGTT	50.0	50.0	57.8	58.2	1474 bp	0.5	57.2
ndhG_p4	ACTTCTGGATTGACAGTGCC	TAGGTGACGAGAATCCCGTT	50.0	50.0	57.8	58.2	1475 bp	0.5	57.2
ndhG_p5	CTTCTGGATTGACAGTGCCA	ATTGATCACATCCGAGCGTT	50.0	45.0	57.8	57.7	1921 bp	0.5	56.0