Hotspot

ndhE

SSC · 115800-116105 · 306 bp

Back to Jacobaea

Pi 0.514

MarkerSeek score 76.70

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 145
pi: 0.514
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TCCTCTGTTCTTGGAGCAGA	TGGAGTAACTTCCGTGGTCT	50.0	50.0	58.0	58.0	1157 bp	0.5	59.8
ndhE_p2	ACCTGTGCCCCAAAATAAGG	TGGAGTAACTTCCGTGGTCT	50.0	50.0	58.1	58.0	1346 bp	0.5	59.5
ndhE_p3	CCTGTGCCCCAAAATAAGGT	TGGAGTAACTTCCGTGGTCT	50.0	50.0	58.1	58.0	1345 bp	0.5	59.5
ndhE_p4	GGCTCAAGTAGGGGTTTCTG	TGGAGTAACTTCCGTGGTCT	55.0	50.0	57.9	58.0	1290 bp	0.5	59.0
ndhE_p5	AGAAAAACCTGTGCCCCAAA	TGGAGTAACTTCCGTGGTCT	45.0	50.0	58.1	58.0	1352 bp	0.5	58.9