Hotspot

ndhA

SSC · 117815-120042 · 2228 bp

Back to Jacobaea

Pi 0.465

MarkerSeek score 81.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2228
variable_sites: 950
pi: 0.465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCAATATACCTTGCGGC	TCAACAGGCCCTAGAAGGAA	50.0	50.0	58.0	58.0	2737 bp	0.5	59.4
ndhA_p2	TGACCAATATACCTTGCGGC	TTCAACAGGCCCTAGAAGGA	50.0	50.0	58.0	58.0	2738 bp	0.5	59.4
ndhA_p3	TGACCAATATACCTTGCGGC	GGCAAAAAGAGGGGGATTCA	50.0	50.0	58.0	58.1	2810 bp	0.5	59.2
ndhA_p4	TGACCAATATACCTTGCGGC	TGGCAAAAAGAGGGGGATTC	50.0	50.0	58.0	58.1	2811 bp	0.5	59.2
ndhA_p5	AAACGCTCGGATGTGATCAA	TCAACAGGCCCTAGAAGGAA	45.0	50.0	57.9	58.0	2824 bp	0.5	58.9