Hotspot

rps15-ycf1

SSC · 121593-121799 · 207 bp

Back to Jacobaea

Pi 0.487

MarkerSeek score 77.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 207
variable_sites: 94
pi: 0.487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCGCAGACCTCTCTGAGATA	TACCGAAACCCCAAAAACGT	55.0	45.0	58.0	58.0	789 bp	0.5	59.4
rps15-ycf1_p2	CCGCAGACCTCTCTGAGATA	AATTATGGAACCTGCCCGTC	55.0	50.0	58.0	57.9	898 bp	0.5	59.4
rps15-ycf1_p3	AACACCATGCATTGATGGGT	TACCGAAACCCCAAAAACGT	45.0	45.0	58.1	58.0	1063 bp	0.5	59.1
rps15-ycf1_p4	AACACCATGCATTGATGGGT	AATTATGGAACCTGCCCGTC	45.0	50.0	58.1	57.9	1172 bp	0.5	59.1
rps15-ycf1_p5	CCGCAGACCTCTCTGAGATA	TATGGAACCTGCCCGTCTAT	55.0	50.0	58.0	57.9	895 bp	0.5	59.0