Hotspot

rpl32-trnL

SSC · 111478-112265 · 788 bp

Back to Jacobaea

Pi 0.482

MarkerSeek score 80.60

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 788
variable_sites: 373
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	TGGCAGCGTTAAAAGCTTTG	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	906 bp	0.5	58.9
rpl32-trnL_p2	TGGCAGCGTTAAAAGCTTTG	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	907 bp	0.5	58.9
rpl32-trnL_p3	GGCAGCGTTAAAAGCTTTGT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	905 bp	0.5	58.9
rpl32-trnL_p4	GGCAGCGTTAAAAGCTTTGT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	906 bp	0.5	58.9
rpl32-trnL_p5	TGGCAGCGTTAAAAGCTTTG	GAAGTAGCAAAGCCTTGGGT	45.0	50.0	57.9	58.1	1413 bp	0.5	58.2