Hotspot

ndhF-rpl32

SSC · 110234-111312 · 1079 bp

Back to Jacobaea

Pi 0.111

MarkerSeek score 79.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1079
variable_sites: 119
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CGCCCACACGATTAGTTACA	TCTGCCCTTACCGAGAAAGA	50.0	50.0	58.0	58.1	2067 bp	0.5	59.5
ndhF-rpl32_p2	CGCCCACACGATTAGTTACA	AAAAAGACTCCCCACCACAC	50.0	50.0	58.0	57.9	2010 bp	0.5	59.5
ndhF-rpl32_p3	GTTGCCGTCGGAAAAAGAAG	TCTGCCCTTACCGAGAAAGA	50.0	50.0	58.0	58.1	1609 bp	0.5	59.2
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	AAAAAGACTCCCCACCACAC	50.0	50.0	58.0	57.9	1552 bp	0.5	59.2
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	TCTGCCCTTACCGAGAAAGA	45.0	50.0	58.0	58.1	1607 bp	0.5	59.1