Hotspot

psbA

LSC · 303-1364 · 1062 bp

Pi 0.00565

MarkerSeek score 47.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 9
pi: 0.00565
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	TGCTTGGGAGTCCCTGATAA	55.0	50.0	58.1	58.0	1336-1363 bp	1.0	82.1
psbA_p2	GATCCACTTGGCTACATCCG	CCTCACGTCGGAAGAAGAAG	55.0	55.0	58.1	58.0	1961-1992 bp	1.0	81.8
psbA_p3	CAATCCACTGCCTTGATCCA	CCTCACGTCGGAAGAAGAAG	50.0	55.0	57.9	58.0	1975-2006 bp	1.0	81.8
psbA_p4	AATCCACTGCCTTGATCCAC	CCTCACGTCGGAAGAAGAAG	50.0	55.0	57.9	58.0	1974-2005 bp	1.0	81.8
psbA_p5	ACAATCCACTGCCTTGATCC	CCTCACGTCGGAAGAAGAAG	50.0	55.0	57.9	58.0	1976-2007 bp	1.0	81.8