Hotspot

petD

LSC · 77225-78438 · 1214 bp

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Pi 0.00494
MarkerSeek score 47.40
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1214
variable_sites
9
pi
0.00494
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 CTACCGTCCAACCGTTACTG AATCAGAATTGCCCCCAAGG 55.0 50.0 58.0 58.1 2245-2266 bp 1.0 80.1
petD_p2 ATTCCTTACCTCGGGACCAA AATCAGAATTGCCCCCAAGG 50.0 50.0 58.0 58.1 2009-2030 bp 1.0 80.0
petD_p3 TCCTTACCTCGGGACCAAAT AATCAGAATTGCCCCCAAGG 50.0 50.0 58.0 58.1 2007-2028 bp 1.0 80.0
petD_p4 CTACCGTCCAACCGTTACTG TGACCAATCAGAATTGCCCC 55.0 50.0 58.0 58.1 2250-2271 bp 1.0 79.9
petD_p5 CTACCGTCCAACCGTTACTG AAGCACTTCATGAAGCCTCC 55.0 50.0 58.0 58.2 2434-2455 bp 1.0 79.7