Hotspot

ccsA-ndhD

SSC · 115923-116191 · 269 bp

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Pi 0.0273
MarkerSeek score 56.60
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
269
variable_sites
9
pi
0.0273
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TATTGGAGTTCCCGGGTCAT GTACTCGGCATTGGCATGTA 50.0 50.0 58.1 58.1 668-686 bp 1.0 80.9
ccsA-ndhD_p2 TATTGGAGTTCCCGGGTCAT CCGCGCGAGTTATTTCTTTC 50.0 50.0 58.1 57.9 707-725 bp 1.0 80.8
ccsA-ndhD_p3 TATTGGAGTTCCCGGGTCAT ATGGGCGGAATAGCTATCCT 50.0 50.0 58.1 58.0 992-1010 bp 1.0 80.5
ccsA-ndhD_p4 TATTGGAGTTCCCGGGTCAT TGACGAAATGGGCGGAATAG 50.0 50.0 58.1 58.1 999-1017 bp 1.0 80.4
ccsA-ndhD_p5 CGAAGCGTGGGGATCATATT ATGGGCGGAATAGCTATCCT 50.0 50.0 57.8 58.0 915-933 bp 1.0 80.2