Hotspot

petD-rpoA

LSC · 78439-78612 · 174 bp

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Pi 0.0728
MarkerSeek score 75.10
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
174
variable_sites
19
pi
0.0728
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 TTGGTTGGGTATTGGTGCAA AATCAGAATTGCCCCCAAGG 45.0 50.0 57.9 58.1 423 bp 1.0 82.4
petD-rpoA_p2 TTTGGTTGGGTATTGGTGCA AATCAGAATTGCCCCCAAGG 45.0 50.0 57.9 58.1 424 bp 1.0 82.4
petD-rpoA_p3 CTCGAGCCGGATGATGAAAA AATCAGAATTGCCCCCAAGG 50.0 50.0 58.1 58.1 913 bp 1.0 80.6
petD-rpoA_p4 CTCGAGCCGGATGATGAAAA TGACCAATCAGAATTGCCCC 50.0 50.0 58.1 58.1 918 bp 1.0 80.4
petD-rpoA_p5 CTCGAGCCGGATGATGAAAA AAGCACTTCATGAAGCCTCC 50.0 50.0 58.1 58.2 1102 bp 1.0 79.9