Hotspot

clpP-psbB

LSC · 72647-73104 · 458 bp

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Pi 0.0109
MarkerSeek score 63.60
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
458
variable_sites
7
pi
0.0109
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGCACTATACGTCAACCCAA CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 550-561 bp 1.0 79.7
clpP-psbB_p2 CGCACTATACGTCAACCCAA GGCCGGGATCATTCAATACA 50.0 50.0 58.0 57.7 582-593 bp 1.0 79.3
clpP-psbB_p3 CGCACTATACGTCAACCCAA ACACGATACCAAGGCAAACC 50.0 50.0 58.0 58.5 553-564 bp 1.0 77.8
clpP-psbB_p4 CGTCAACCCAAGATGCATCT CGATACCAAGGCAAACCCAT 50.0 50.0 58.3 58.2 541-552 bp 1.0 77.7
clpP-psbB_p5 CGTCAACCCAAGATGCATCT GGCCGGGATCATTCAATACA 50.0 50.0 58.3 57.7 573-584 bp 1.0 77.3