Hotspot

psbE-petL

LSC · 65389-66449 · 1061 bp

Pi 0.011

MarkerSeek score 64.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1061
variable_sites: 17
pi: 0.011
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACGAATAGCCAACCAGCAAT	AGCCGTATCTTGTTCAAACCA	45.0	42.9	57.9	57.9	1262-1267 bp	1.0	71.1
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGCCGTATCTTGTTCAAACCA	55.0	42.9	58.2	57.9	1171-1176 bp	1.0	70.6
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AGCCGTATCTTGTTCAAACCA	50.0	42.9	58.4	57.9	1169-1174 bp	1.0	69.4
psbE-petL_p4	CGAATAGCCAACCAGCAATG	AGCCGTATCTTGTTCAAACCA	50.0	42.9	57.5	57.9	1261-1266 bp	1.0	68.3
psbE-petL_p5	AGCAAAAGAGCGTTCTCCTG	AGCCGTATCTTGTTCAAACCA	50.0	42.9	58.5	57.9	1182-1187 bp	1.0	68.3